Super resolution microscopy station on the OCTOPUS facility
The latest addition to the CLF’s
OCTOPUS (link opens in a new window)imaging facility is a super-resolution microscopy station, capable of achieving 50 nm resolution in 2D.
The microscope is dedicated to 2D fluorophore localisation methods of super-resolution microscopy, such as stochastic optical reconstruction optical microscopy (STORM) and photoactivation localisation microscopy (PALM) and is coupled to 100 mW excitation and activation lasers at 405, 488, 561 and 642 nm.
The principal difference between this microscope and the other single molecule microscopes is that the power at the sample plane is much greater, both because the laser is coupled to the microscope through free space and because it is focused to a smaller spot in the sample plane, ensuring that the incident intensity is suited to super-resolution imaging.
Proposals for access are now being accepted for this microscopy station through the CLF access scheme (link opens in a new window). Please click here (link opens in a new window) more details of the Lasers for Science application procedure.
