Using single-molecule and super-resolution fluorescence microscopy techniques to observe the responses of the Epidermal Growth Factor (EGF) family of Receptors to stimuli. Primarily interested in applying FLImP and STORM techniques to assess the nano-scale conformational changes of the extracellular and kinase domains of EGF Receptors during signalling in cells. With the help of molecular dynamic simulations, to obtain a picture of the entire membrane-embedded EGF receptor, studying the intra-protein structural changes and the alternative receptor-receptor interactions that occur during aberrant signalling in cancerous cells and in cells expressing mutated receptors (naturally occurring and purposefully designed mutations). Perturbing cellular systems to identify the effects of therapeutically-relevant small molecule inhibitors and antibodies on signalling mechanisms.

Background

PhD in Biochemistry - University of Leicester

BSc Medical Biochemistry – University of Birmingham

General

Selene joined STFC in 2005 and the LSF at the Rutherford Appleton Laboratory in 2008. Her research aims to combine many scientific tools to advance understanding within the area of cancer cell biology. In particular she questions the structure-function relationship of proteins in their natural environment by developing cell and molecular biology methods, protein biochemistry, fluorescent labelling of ligands and antibodies and multi-dimensional and multi-colour super-resolution imaging. Selene is also a co-principle investigator of the grant entitled “Investigating the role of the architecture of EGFR oligomers in lung cancer: A single molecule super-resolution approach to the development of targeted therapies".

 

For publications please see: https://www.researchgate.net/profile/Selene_Roberts​