For more information please contact: Dr. Andy Ward.
Technique and applications
| Specifications
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Technique:
Laser capture of microscopic particles to measure forces, Raman spectroscopy and Mie scattering. Combined with imaging techniques of TIRF, confocal, multiphoton FLIM and PLIM
Applications:
Colloid and interface science, medical research, atmospheric chemistry, aerosol science, material science, biological research, plant cell studies, catalysis.
| Lasers: Laser trapping: 488 nm, 514.5 nm, 532 nm, 700 – 900 nm, 1064 nm, 1090 nm. Raman spectroscopy : 488 nm, 514.5 nm, 532 nm, 785 nm and 1064 nm.
Broadband Mie scattering: 400 nm to 1700 nm. Detectors: 450 – 700 nm and 600 - 1000 nm Acton spectrographs\PI Spec 10 detectors
900 – 1700 nm Isoplane spectrograph\PI NIRvana detector. Objectives: 100x and 63x oil immersion, 63x water immersion. 100x and 50x long working distance air.
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The capture of microscopic particles with laser light can be performed with single laser beams using high numerical aperture objectives (Laser Tweezers), or with lower numerical aperture optics where another force is present such as gravity, an interface or an opposing laser (Laser Trapping). We offer both trapping techniques and combine these with other imaging techniques to meet experimental requirements. Typical combinations are with Force measurement, Raman spectroscopy, Mie scattering spectroscopy, Confocal microscopy and Fluorescent Lifetime Imaging microscopy. For Raman spectroscopy we frequently, but not exclusively, use the trapping wavelength as the Raman probe.
Samples are typically polymer beads, emulsions, bacteria and cells dispersed in liquids. We can also offer airborne trapping of droplets and solids below 10 microns diameter and options for atomisation and nebulisation.
Microscope Full Specifications
| Instrument | Optical trapping and Raman spectroscopy |
| Laser wavelengths | 488, 514.5, 532, 700-900, 1064 nm |
| Compatible objective lenses | 100x oil immersion, 63x oil immersion, 63x water immersion. Long working distance 100x and 50x air. |
| Raman spectroscopy ranges | 450 – 700 nm Acton spectrograph\PI Spec 10 detector
600 – 1000 nm Acton spectrograph\PI Spec 10 detector
900 – 1700 nm Isoplane spectrograph\PI NIRvana detector |
| Temperature control | 5-90 °C |
Applications
Colloid and interface science, medical research, atmospheric chemistry, aerosol science, material science, biological research, plant cell studies, catalysis.
Case Studies
