Multimodality Optical NanoScopy TERminus (MONSTER)

10 Jul 2019

Yes

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About

The MONSTER microscope is a combined ZEISS LSM880-Elyra PS1 system in which simultaneous multi-colour imaging is enabled thanks to the dual EMCCD camera setting.​ It incorporates multiple imaging modalities shown below.

Please contact Dr. Lin Wang​ for further details.

​​​Technique and applications	​Specifications
​Technique: Confocal, AiryScan, Structured Illumination Microscopy, Single Molecule Localisation Microscopy (PALM/STORM). Applications: Biolog​​ical and medical research, material science, chemistry	​Lasers: Multicolour imaging with 405, 458, 488, 514, 561, 6330 nm excitation. Detectors: Dual EMCCD, AiryScan detector. Objectives: 63x oil and water immersion,100x oil and silicone immersion.

Example data​​

​​Simultaneous two-colour SIM imaging of CHO cells expressing alpha-1 an​titrypsin-TMR (red) and ER membra​ne-GFP (blue). Frame rate 0.6 ​​fps (10X speed up in t​he video), resolution in SIM ~100 ​nm. Sample courtesy Joe Chambers at Uni of Cambridge.​

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Microscope full specifications

​Technique	Confocal	Airyscan	SIM	SMLM (PALM/STORM)
Laser wavelengths (nm)	405, 458, 488, 514, 561, 633	405, 458, 488, 514, 561, 633	405, 488, 561, 642	405, 488, 561, 642
Simultaneous dual colour imaging	Yes	No	Yes	Yes
Compatible objective lenses	C-Apo 40x/1.20 w, Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil)	C-Apo 40x/1.20 w, Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil)	Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil)	Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil)
Field of View (‎μm)	135×135 (63x/1.40 NA objective)	135×135 (63x/1.40 NA objective, Fast mode)	80×80 (63x/1.40 NA objective)	14×14 (100x/1.46 NA objective, µHP mode)/26x26 (HP mode)
Lateral resolution (nm) (λ=488nm)	240 (63x/1.40 NA objective)	140(normal mode)/120(2D SR or 3D mode)/145 in X, 180 in Y (Fast SR mode)	120	20 (localisation precision)
Axial resolution (nm) (λ=488nm)	700 (63x/1.40 NA objective)	350 (normal mode)/ 450 (Fast SR mode)	300	50
One shot 3D method	N/A	N/A	N/A	phase ramp, 1µm depth
Sample thickness (‎μm)	100	100	20	10
Temporal resolution	0.85 fps at 480x480 pixels	27 fps at 480x480 pixels in Fast mode	1.6 second per frame (at 24 ms integration time)	several minutes

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