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Marisa Martin-Fernandez publications
21 Nov 2022
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​Publicati​ons​
  1. ​​A perspective of fluorescence microscopy for cellular structural biology with EGFR as witness.​ Martin-Fernandez, M. L. Journal of Microscopy, 00, 1– 19. https://doi.org/10.1111/jmi.13151​​​​
  2. Yanan Zhu, Christopher W. Koo, C. Keith Cassidy, Matthew C. Spink, Tao Ni, Laura C. Zanetti-Domingues, Benji Bateman, Marisa L. Martin-Fernandez, Juan Shen, Yuewen Sheng, Yun Song, Zhengyi Yang, Amy C. Rosenzweig & Peijun Zhang.  Structure and activity of particulate methane monooxygenase arrays in methanotrophs. Nat Comms. (2022)​, 13, 5221 . https://doi.org/10.1038/s41467-022-32752-9
  3. C. Qiu, Y. Odarchenko, O. Meng, S. Xu, I. Lezcano-Gonzalez, P. Olalde-Velasco, F. Maccherozzi, L.C. Zanetti Domingues, M.L. Martin-Fernandez & A. Beale. Resolving the effect of oxygen vacancies on Co nanostructures using soft XAS/X-PEEM. ACS Catal. (2022), 12, 15, 9125–913,4 https://doi.org/10.1021/acscatal.2​c00611.​
  4. V. Jackson, J. Hermann, C.J. Tynan, D. J. Rolfe, R. Corey, A. Duncan, M. Noriega, A. Chu, A. Kalli, E. Y. Jones, M. Sansom, M. L. Martin-Fernandez, E. Seiradake, & M. Chavent. The guidance and adhesion protein FLRT2 dimerizes in cis via dual Small-X3-Small transmembrane motifs. Structure (2022) https://doi.org/10.1016/j.str.2022.05.014
  5. A. dos Santos, Natalia Fili; Yukti Hari-Gupta, Rosemarie E Gough, Lin Wang, Marisa L. Martin-Fernandez, Jesse Arron, Eric Wait, Teng Leong Chew, and Chris Toseland. Binding partners regulate unfolding of myosin VI to activate the molecular motor. Biochem. J. (2022) BCJ20220025. https://doi.org/10.1042/BCJ20220025
  6. Y. Hari-Gupta, N. Fili, Á. dos Santos, A. W. Cook, R. E. Gough, H. C. W. Reed, L. Wang, J. Aaron, T. Venit, E. Wait, A. Grosse-Berkenbusch, J. C. M. Gebhardt, P. Percipalle, T-L. Chew, M.L. Martin-Fernandez, C. P. Toseland Myosin VI regulates the spatial organization of mammalian transcription initiation. Nat. Comms. (2022) https://doi.org/10.1038/s41467-022-28962-w (selected as a preprint highlight by PreLights)
  7. Á. dos Santos, D. E. Rollins, Y. Hari-Gupta, H. C. W. Reed, M. Du, S. Yong Zi Ru, K. Pidlisna, A. Stranger, F. Lorgat, I. Brown, K. Howland, J. Aaron, L. Wang, P. J. I. Ellis, T.L. Chew, M.L. Martin-Fernandez, A. L. B. Pyne,  C. P. Toseland. Autophagy receptor NDP52 alters DNA conformation to modulate RNA Polymerase II transcription. Submitted Nat. Comms. (2022) https://doi.org/10.1101/2022.02.01.478690
  8. M.L. Martin-Fernandez. Fluorescence Imaging of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Resistance in Non-Small Cell Lung Cancer. Cancers (2022) 14(3), 686; https://doi.org/10.3390/cancers14030686.
  9. L. Mendonça, A. Howe, J.B. Gilchrist, Y. Sheng, D. Sun, M.L. Knight, L.C. Zanetti-Domingues, B. Bateman, A. Krebs, L. Chen, J. Radecke, V. D. Li, T. Ni, I. Kounatidis, M. A. Koronfel, M. Szynkiewicz, M. Harkiolaki, M. L. Martin-Fernandez, W. James & P. Zhang Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress. Nat. Comms. (2021) 12, 4629. https://doi.org/10.1038/s41467-021-24887-y
  10. Y. Zhu, D. Sun, A.s Schertel, J. Ning, Xi.Fu, P.P. Gwo, A.M. Watson, Z.Freyberg, L. Zanetti-Domingues, M.L. Martin-Fernandez, and  P. Zhang. Serial cryoFIB/SEM Reveals Cytoarchitectural Disruptions in Leigh Syndrome Patient Cells. Structure (2021) https://doi.org/10.1016/j.str.2020.10.003.
  11. I. Emmanouilidis,  N. Fili,  A.W. Cook, Y. Hari-Gupta, A dos Santos, L. Wang, M. L. Martin-Fernandez, P. J. I. Ellis   and C. P. Toseland. A Targeted and Tuneable DNA Damage Tool Using CRISPR/Cas9. Biomolecules (2021) 11, 288. https://doi.org/10.3390/biom11020288.
  12. L. C. Zanetti-Domingues, S.E. Bonner, S. Iyer, M.L. Martin-Fernandez and V. Huber. Cooperation and interplay between EGFR signalling and Extracellular Vesicle Biogenesis in Cancer. Cells (2020) 9, 2639; DOI:10.3390/cells9122639.
  13. L.C. Zanetti-Domingues, S.E. Bonner, M.L. Martin-Fernandez, and V. Huber. Mechanisms of Action of EGFR Tyrosine Kinase Receptor Incorporated in Extracellular Vesicles. Cells (2020) 9(11), 2505. DOI: 10.3390/cells9112505.
  14. Á. dos Santos, A.W. Cook, R.E. Gough, M. Schilling, N.A. Olszok, I. Brown, L. Wang, J.A, M.L. Martin-Fernandez, F. Rehfeldt, and C.P. Toseland. DNA damage alters nuclear mechanics through chromatin reorganisation. Nucleic Acids Research (2020) DOI: 10.1093/nar/gkaa1202.
  15. M.L. Martin-Fernandez. A brief​​ history of the Octopus Imaging Facility to celebrate its 10th anniversary. J. Microscopy (2020) DOI: 10.1111/jmi.12974.
  16. B. Ambrose, J. Baxter, J. Cully, M. Willmot, E. Steele, B. Bateman, M.L. Martin-Fernandez, A. Vabdy, M. Aaldering, and T.D. Craggs. The smfBox: an open-source platform for single-molecule FRET. Nat. Comms. (2020) 11, 5641. DOI: 10.1038/s41467-020-19468-4.
  17. A. Danson, A. McStea, L. Wang, A.Y. Pollitt, M.L. Martin-Fernandez, I. Moraes, M.A. Walsh, S. MacIntyre, K.A. Watson. Super-resolution fluorescence microscopy reveals clustering behaviour of Chlamydia pneumoniae's Major Outer Membrane Protein. Biology J. (2020) 9(10), 344; https://doi.org/10.3390/biology9100344.
  18. A. Colomba; M. Fitzek; R. George; G. Weitsman; S. Roberts; L. Zanetti-Domingues; M. Hirsch; D.J. Rolfe; S. Mehmood; A. Madin; J. Claus; S. Kjaer; A.P. Snijders; T. Ng; M.L. Martin-Fernandez; D. M. Smith; P.J. Parker. A small molecule inhibitor of HER3: a proof-of-concept study. Biochem. J. (2020) 477 (17): 3329–3347. https://doi.org/10.1042/BCJ20200496.
  19. S.C. Salvage, J.S Rees, A. McStea, M. Hirsch, L. Wang, C.Tynan, M.W. Reed, J.R. Irons, R. Butler, A. J. Thompson, M.L. Martin-Fernandez, Christopher LH. Huang, Antony P. Jackson. Supramolecular clustering of the cardiac sodium channel Nav1.5 in HEK293 cells, with and without the auxiliary β3 subunit. Faseb J. (2020) DOI: 10.1096/fj.201701473RR.
  20. N. Fili, Y. Hari-Gupta, B. Aston, A. dos Santos, R.E. Gough, B. Alamad, L. Wang, M.L. Martin-Fernandez and  C.P. Toseland. Competition between two high- and low-affinity protein-binding sites in myosin VI controls its cellular function.  J. Biol. Chem.(2020) DOI: 10.1074/jbc.RA119.010142.
  21. J. Periz, M. Del Rosario, A. McStea, S. Gras, C. Loney, L. Wang, M.L. Martin-Fernandez and M. Meissner. A highly dynamic F-actin network regulates transport and recycling of micronemes in Toxoplasma gondii vacuoles. Nat. Comms. (2019) DOI: 10.1038/s41467-019-12136-2.
  22. M. Hirsch, R. Wareham, J. W. Yoon, D. J. Rolfe, L. C. Zanetti-Domingues, M. P. Hobson, P. J. Parker, M. L. Martin-Fernandez, S. Singh.  A global sampler of single particle tracking solutions for single molecule microscopy. PLoS One (2019) DOI: 10.1371/journal.pone.0221865.
  23. J.F. McKenna, D.J. Rolfe, A.F. Tolmie, S.W. Botchway, M.L. Martin-Fernandez, C. Hawes, J. Runions. The cell wall regulates dynamics and size of plasma-membrane nanodomains in Arabidopsis. PNAS (2019) https://doi.org/10.1073/pnas.1819077116.
  24. B. Bateman, L. C. Zanetti-Domingues, A. N. Moores, S. R. Needham, D. J. Rolfe, L. Wang, D. T. Clarke and M. L. Martin-Fernandez. Super-resolution Microscopy at Cryogenic Temperatures Using Solid Immersion Lenses. Bio-Protocol (2019) DOI: 10.21769/BioProtoc.3426.
  25. M.L. Martin-Fernandez, D.T. Clarke, S.K. Roberts, L.C. Zanetti-Domingues and F.L. Gervasio. Structure and Dynamics of the EGF Receptor as Revealed by Experiments and Simulations and its Relevance to Non-Small Cell Lung Cancer. Cells (2019) 8(4) DOI: 10.3390/cells8040316.)
  26. A. Elbediwy, Y. Zhang, M. Cobbaut, P. Riou, R.S. Tan, S.K. Roberts, C. J.Tynan, R. G. S. Kjaer, M.L. Martin-Fernandez, B.J. Thompson, N.Q. McDonald and P.J. Parker. The Rho-family GEF FARP2 is activated by aPKCι to control polarity and tight junction formation. J. Cell Science (2019) 132(8) DOI: 10.1242/jcs.223743.
  27. D.T. Clarke & M.L. Martin-Fernandez. A Brief History of Single-Particle Tracking of the Epidermal Growth Factor Receptor. Methods & Protocols (2019) 2, 12; DOI:10.3390/mps2010012.
  28. L. Wang, B. Bateman, L. C. Zanetti-Domingues, A. N. Moores, S. Astbury, C. Spindloe, M. C. Darrow, M. Romano, S. R. Needham, K. Beis, D. J. Rolfe, D. Clarke and M. L. Martin-Fernandez. Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution. Comms. Biol. (2019) Vol 2. doi.org/10.1101/373647.
  29. S.K. Roberts, M. Hirsch, Alexandra McStea, Laura C. Zanetti-Domingues, Jeroen Claus, Peter J. Parker, Lin Wang & Marisa L. Martin-Fernandez. Cluster analysis of endogenous HER2 and HER3 receptors in SKBR3 cells. Bio-protocol (2018) DOI:   10.21769/BioProtoc.3096.
  30. L.C. Zanetti-Domingues, D. Korovesis, S.R. Needham, C.J. Tynan, S. Sagawa, S.K. Roberts, A. Kuzmanic, E. Ortiz-Zapater, P. Jain, R.C. Roovers, A. Lajevardipour, P.M.P. van Bergen en Henegouwen, G. Santis, A.H.A. Clayton, D.T. Clarke, F.L. Gervasio, Y. Shan, D.E. Shaw, D.J. Rolfe, P.J. Parker, and M.L. Martin-Fernandez. The architecture of EGFR's basal complexes reveals autoinhibition mechanisms in dimers and oligomers. Nat. Comms. (2018); DOI:10.1038/s41467-018-06632-0.
  31. D.T. Clarke, L.C. Zanetti-Domingues and M.L. Martin-Fernandez. High‐Resolution Microscopy for Structural Studies of Biological Systems in Cells. eLS (2018); pp.1-10 DOI: 10.1002/9780470015902.a0027945
  32. J. Claus, G. Patel, F. Autore, A. Colomba, G. Weitsman, F. N Soliman,  S.K. Roberts, L.C. Zanetti-Domingues, M. Hirsch, F. Collu, R. George, E. Ortiz-Zapater, P.R. Barber, B. Vojnovic, Y. Yarden, M.L. Martin-Fernandez,   A. Cameron,  F. Fraternali, T. Ng  and P.J. Parker. Inhibitor-induced HER2-HER3 heterodimerisation promotes proliferation through a novel dimer interface.  eLife (2018) DOI: 10.7554/eLife.32271.
  33. M. L. Martin-Fernandez, L. C. Zanetti-Domingues, D. Korovesis, S. R. Needham, C. J. Tynan, S. K. Roberts, D. T. Clarke, D.l J. Rolfe, P. J. Parker. Fluorescence Localisation Imaging with Photobleaching at 5 nm Resolution Reveals the Architecture of Basal EGFR Complexes and Mechanisms of Autoinhibition and Activation. Biophys. J. (2018) 114(3):201a-202a, doi.org/10.1016/j.bpj.2017.11.1126.
  34. Tiede C., Bedford R., Heseltine S.J., Smith G., Wijetunga I., Ross R., AlQallaf D., Roberts A.P., Balls A., Curd A., Hughes R.E, Martin H., Needham S.R., Zanetti-Domingues L.C., Sadigh Y., Peacock T.P., Tang A.A., Gibson N., Kyle H., Platt G.W., Ingram N., Taylor T., Coletta L.P., Manfield I., Knowles M., Bell S., Esteves F., Maqbool A., Prasad R.K, Drinkhill M., Bon R.S., Patel V., Goodchild S.A., Martin-Fernandez M.L., Owens R.J., Nettleship J.E., Webb M.E., Harrison M., Lippiat J.D., Ponnambalam S., Peckham M., Smith A., Ferrigno P.K., Johnson M., McPherson M.J., Tomlinson D.C. Affimer proteins are versatile and renewable affinity reagents.  eLife (2017) DOI: 10.7554/eLife.24903.
  35. Needham, S.R, Zanetti-Domingues, L.C., Arkhipov, A., Mysore, V., Korovesis, D., Roberts, S.K., Tynan, C. J., Rolfe, D. J., Hirsch, M., Lajevardipour, A., Clayton, A.H.A., Parker, P.J., Shan, Y.E. Shaw, D. and Martin-Fernandez, M. L. Oligomerization of the Epidermal Growth Factor Receptor Organizes Kinase-Active Dimers into Competent Signaling Platforms. Biophys. J. (2017) 112. 26a-27a. 10.1016/j.bpj.2016.11.180. (IF 4)
  36. Needham, S.R., Roberts, S.K., Arkhipov, A., Mysore, V.P., Tynan, C.J., Zanetti-Domingues, L.C., Kim, E.T, Losasso, V., Korovesis, D., Hirsch, M., Rolfe, D.J., Clarke, D.T., Winn, M., Lajevardipour, A., Clayton, A.H.A. Pike, L.J., Perani, M., Parker, P.J., Shan, Y, Shaw, D.E. & Martin-Fernandez, M.L. Oligomerization of the Epidermal Growth Factor Receptor Organizes Kinase-Active Dimers into Competent Signalling Platforms. Nat. Comms. (2016) DOI: 10.1038/ncomms13307.
  37. Coles, B.C., Webb, S.E.D., Schwartz, N., Rolfe, D.J. Martin-Fernandez, M.L., and Valentina Lo Schiavo. Characterisation of the effects of optical aberrations in single molecule techniques. Biomed. Opt. Express (2016) DOI:10.1364/BOE.7.001755.
  38. C. J. Tynan, V. Lo Schiavo, L. Zanetti-Domingues, S.R. Needham, S. K. Roberts, M. Hirsh, D. J. Rolfe, D. Korovesis, D. T. Clarke and M. L. Martin-Fernandez.  A tale of the epidermal growth factor receptor: The quest for structural resolution on cells. Methods (2015) DOI: 10.1016/j.ymeth.2015.10.009.
  39. Stephen E D Webb, Michael Hirsch, Sarah R Needham, Benji  C Coles, Selene K Roberts, Laura C Zanetti-Domingues, Chris J Tynan, Marisa L. Martin-Fernandez and Daniel J Rolfe. Nanometric molecular separation measurements by single molecule photobleaching. Methods (2015) DOI: 10.1016/j.ymeth.2015.05.009.
  40. Laura C. Zanetti-Domingues, Michael Hirsch, Christopher J. Tynan, Daniel J. Rolfe, Teodor Boyadzhiev, Kathrin Scherer, David T. Clarke, Marisa L. Martin-Fernandez, and Sarah R. Needham. Determining the geometry of oligomers of the human epidermal growth factor family on cells with 7 nm resolution. Progress in Biophys & Mol Biol. (2015) DOI:10.1016/j.pbiomolbio.2015.04.002.
  41. O. Coban, L. Zanetti-Dominguez, D. R. Matthews, D. J. Rolfe, G. Weitsman, P.R. Barber, J. Barbeau, V. Devauges, F. Kampmeier, M. Winn, Bo. Vojnovic, P. J. Parker, K. A. Lidke, D. S Lidke, S. M. Ameer-Beg, M. L. Martin-Fernandez and T. Ng. Effect of phosphorylation on the EGFR dimer stability probed by single-molecule dynamics and FRET/FLIM. Biophys. J. (2015), 108 (5), 1013-1026. DOI: 10.1016/j.bpj.2015.01.005.
  42. Sarah R. Needham, Laura C. Zanetti-Domingues, Kathrin Scherer, Michael Hirsch, Daniel J. Rolfe, Selene K. Roberts, Marisa L. Martin-Fernandez, David T. Clarke, Christopher J. Tynan. Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution.  Biochem. Soc. Trans. (2014) (3):309-14. DOI: 10.1042/BST20140318.
  43. T. Kiuchi, E. Ortiz-Zapater, J. Monypenny, D. R. Matthews, J. Barbeau, O. Coban, K. Lawler, B. Burford, D. Rolfe, E. de Rinaldis, D. Dafou, M. A. Simpson, N. Woodman, S. Pinder, C.E. Gillett, V. Devauges, S. P. Poland, G. Fruhwirth, P. Marra, Y. L. Boersma, A. Plückthun, W.J Gullick, Y. Yarden, G.Santis, M. Winn, M. L. Martin-Fernandez, P. Parker, A. Tutt, S. M. Ameer-Beg, Tony Ng. The ErbB4 CYT2 variant protects EGFR from ligand-induced degradation to enhance cancer cell motility. Sci. Signal. (2014) Vol. 7, Issue 339, p. ra78 DOI: 10.1126/scisignal.2005157.
  44. Needham, S.R; Zanetti-Domingues, L.C; Hirsch, M., Rolfe, D. J; Tynan, C.J; Roberts, S K; Martin-Fernandez, M.L; Clarke, David T. Structure-function relationships and supramolecular organization of the EGFR (epidermal growth factor receptor) on the cell surface. Biochem. Soc. Trans. (2014) 42 Issue:1: 114-9 DOI:10.1042/BST20130236.
  45. O. Coban, D. R. Matthews, S. M. Ameer-Beg, D. J. Rolfe, G. Weitsman, F. Kampmeier, M. Winn, L. Zanetti-Dominguez, M. L. Martin-Fernandez and T. Ng. Modulation of EGFR Dimer Stability by Manipulation of Phosphorilation in Situ. Biophys. J.(2014) https://doi.org/10.1016/j.bpj.2013.11.628.
  46. Laura C. Zanetti-Domingues., Christopher J. Tynan., Daniel J. Rolfe, David T. Clarke, Marisa L. Martin-Fernandez. Hydrophobic Fluorescent Probes Introduce Artifacts into Single Molecule Tracking Experiments Due to Non-Specific Binding. PLoS One (2013) DOI: 10.1371/journal.pone.0074200.
  47. M.L. Martin-Fernandez, C.J. Tynan & S.E.D. Webb. A 'Pocket Guide' to Total Internal Reflection Fluorescence.  J. Microscopy (2013) 252(1):16-22 DOI: 10.1111/jmi.12070.
  48. S.R. Needham, M. Hirsch, D.J. Rolfe, D. T Clarke, L. C Zanetti-Domingues, R. Wareham, and M.L Martin-Fernandez. Measuring EGFR separations on cells with ~10 nm resolution via fluorophore localization imaging with photobleaching. PLoS One (2013) 8(5):DOI:0.1371/journal.pone.0062331.
  49. S.E.D. Webb, S. Needham, S.K. Roberts, M. Hirsch, C.J. Tynan, B.C. Bateman, D.T. Clarke, L. Zanetti-Domingues, D. Korovesis, and M. L. Martin-Fernandez. Nanoscale EGFR separations in cells using fluorophore localisation confidence intervals.  Eur. Biophys. J. (2013) Volume: 42: S54-S54.
  50. Hirsch, M., Wareham RJ, Martin-Fernandez M.L., Hobson M.P. and Rolfe D.J. A Stochastic Model for Electron Multiplication Charge-Coupled Devices - From Theory to Practice. PLoS One (2013) 8(1): e53671. DOI:10.1371/journal.pone.0053671.
  51. L.C. Zanetti-Domingues, S.E.D. Webb, C. J. Tynan, D. J. Rolfe, M. Hirsch, B. Coles, S.R. Needham,  S. K. Roberts, D.T. Clarke and M. L. Martin-Fernandez. Simultaneous, multicolour single molecule imaging of the entire human epidermal growth factor receptor (HER) family in live breast cancer cells. Annals of Oncology (2012) doi.org/10.1093/annonc/mdt086.3.
  52. M. L. Martin-Fernandez and David T. Clarke. Single Molecule Fluorescence Detection and Tracking in Mammalian Cells: The State-of-the-Art and Future Perspectives. Special Issue "Advances in Single Molecule Spectroscopy" - Int. J. Mol. Sci. (2012) DOI:10.3390/ijms131114742.
  53. Zanetti-Domingues, L.C., Martin-Fernandez, M.L., Needham S.R, D.J Rolfe and D.T. Clarke. A systematic investigation of differential effects of cell culture substrates on the extent of artifacts in single-molecule tracking. PLoS One (2012) 7(9): e45655. DOI:10.1371/journal.pone.0045655.
  54. Martiniere, I. Lavagi, G. Nageswaran, D.J Rolfe, L. Maneta-Peyret, D-T. Luu, S. W Botchway, S.E D Webb, S. Mongrand, C. Maurel, M.L Martin-Fernandez, J. Kleine-Vehn, J. Friml, P. Moreau, and J. Runions. The cell wall constrains lateral diffusion of plant plasma-membrane proteins. PNAS (2012) pnas.1202040109.
  55. C.J. Tynan, David T. Clarke, Benjamin C. Coles, M.L. Martin-Fernandez and S.E. D. Webb. Multicolour Single Molecule Imaging with Near Infra-red Dyes. PLoS ONE (2012) 7(4): e36265. DOI:10.1371/journal.pone.0036265
  56. S.E.D. Webb, D.J. Rolfe, B.C. Coles, R. Wareham and M.L. Martin-Fernandez. Multicolour single molecule imaging in cells using a supercontinuum source. Biomed. Opt. Express (2012) Exp. Vol. 3, No. 3: 400-406. https://doi.org/10.1364/BOE.3.000400
  57. M.L. Martin-Fernandez. Human Epidermal Growth Factor Receptor (HER1) aligned on the plasma Membrane Adopts Key Features of Drosophila EGFR Asymmetry. Biochem. Soc. Trans. (2012) 40(1):184-188  DOI: 10.1042/BST20110692
  58. S. K. Roberts, C.J. Tynan, M. Winn, and M.L. Martin-Fernandez. Investigating extracellular in situ EGFR structure and conformational changes using FRET microscopy. Investigating extracellular in situ EGFR structure and conformational changes using FRET microscopy. Biochem. Soc. Trans. (2012) 40(1):189-194. https://doi.org/10.1042/BST20110632.
  59. D.J. Rolfe, C.I. McLachlan, M. Hirsch, S.R. Needham, C.J. Tynan, S. E. D. Webb, M. L. Martin-Fernandez and M.l.P. Hobson. Automated multidimensional single molecule fluorescence microscopy feature detection and tracking. Eur. Biophys. J. (2011) 40:1167–1186. https://doi.org/10.1007/s00249-011-0747-7.
  60. D.T. Clarke, S.W. Botchway, B.C. Coles, S. R. Needham, S.K. Roberts, D.J. Rolfe, C.J. Tynan, A.D. Ward, S.E.D. Webb, R. Yadav, L. Zanetti-Domingues, and M.L. Martin-Fernandez. Optics clustered to output unique solutions: A multi-laser facility for combined single molecule and ensemble microscopy. Rev. Sci. Ins. (2011) 82, Article Number: 093705. DOI: 10.1063/1.3635536.
  61. M.L. Martin-Fernandez, David T. Clarke, Sarah R. Needham, Selene K. Roberts, Daniel J. Rolfe, Chris J. Tynan, Stephen E.D. Webb and Martyn Winn. Investigating the conformation of membrane proteins in cells via single molecule microscopy. Molecular Imaging ISBN (2011) 979-953-307-076-5.
  62. G. Vizcay Barrena, S.E.D. Webb, M.L. Martin-Fernandez, and Z.A. Wilson. Subcellular and single-molecule imaging of plant fluorescent proteins using total internal reflection fluorescence microscopy (TIRFM) J. Exp. Bot.  (2011) 62 (15): 5419-5428.
  63. F. Hussain, M. Parsons, M. Snippe, C. Farmer, P. E. Morton, M.L. Martin-Fernandez and George Santis. CAR Modulates E-Cadherin Dynamics in the Presence of Adenovirus Type 5. PLoS ONE  (2011) 6 (8):  Article Number: e23056.
  64. C.J. Tynan, S.K. Roberts, D.J. Rolfe, D.T. Clarke, H.H. Loeffler, J. Kästner, M.D. Winn P.J. Parker and M.L. Martin-Fernandez. Human epidermal growth factor receptor (EGFR) aligned on the plasma membrane adopts key features of drosophila EGFR asymmetry. Mol. Cell Biol. (2011) 31(11):2241-2252
  65. S. E. D. Webb, C.J. Tynan, L. Zanetti-Domingues, B.C. Coles, S.K. Roberts, D.J. Rolfe, S. R. Needham, M. Hirsch, D.T. Clarke, and M.L. Martin-Fernandez. Simultaneous, multicolour single molecule imaging of the entire ErbB receptor family in live cells. Eur. Biophys. J. with Biophys. Lett. (2011) 40(1): 154.
  66. Askari J.A., Tynan C.J., Webb S.E.D., Martin-Fernandez M.L., Ballestrem C., Humphries M.J. Focal adhesions are sites of integrin extension. J. Cell Biology (2010) 188(6):891-903.
  67. Ismail T.M., Zhang S., Fernig D.G., Gross S., Martin-Fernandez M.L., See V., Tozawa K, Tynan C.J., Tynan, C.J., Wang G.Z., Wilkinson M.C., Rudland P.S. and Barraclough R. Self-association of Calcium-binding Protein S100A4 and Metastasis. J. Biol. Chem. (2010) 285(2):914-922.
  68. Askari, J. A., Tynan, C. J., Webb, S. E., Martin-Fernandez, M. L., Ballestrem, C., Humphries, M. J.. Imaging integrin alpha 5 beta 1 conformation on adherent cells' International J. Exp. Path. (2010) 91 (6)
  69. Kastner J., Loeffler H.H., Roberts S.K., Martin-Fernandez M.L., and Winn M.D. (2009). Ectodomain orientation, conformational plasticity and oligomerization of ErbB1 receptors investigated by molecular dynamics. J. Structural Biology (2009) 167(2):117-128.
  70. S.E.D. Webb, D.J. Rolfe, S.R. Needham, S. K. Roberts, D.T. Clarke, C.I. McLachlan, M.P. Hobson and M.L. Martin-Fernandez. Simultaneous widefield single molecule orientation and FRET microscopy in cells. Optics Express (2008) 16(25): 20258-20265.
  71. S.E.D. Webb, S. K. Roberts, S.R. Needham, C.J. Tynan, D.J. Rolfe, M.D. Winn, R. Barraclough, D.T. Clarke, and M.L. Martin-Fernandez. Single molecule imaging and FLIM show different structures for high and low-affinity EGFRs in A431 cells. Biophysics Journal (2008) 94:803-819.
  72. Stephen E. D. Webb, Selene K. Roberts, Sarah R. Needham, Daniel J. Rolfe, Chris J. Tynan, and M.L. Martin-Fernandez. Epidermal growth factor signalling studied using multidimensional single molecule fluorescence microscopy. Proc. SPIE (2007) 6441, 64410E.
  73. S.E.D. Webb, S.R. Needham, S.K. Roberts, and M.L. Martin-Fernandez. Multidimensional single molecule imaging in live cells using total internal reflection fluorescence microscopy. Optics Letters (2006) 15;31(14):2157-9.
  74. S. Zhang, G. Wang, D.G. Fernig, P.S. Rudland, S.E.D. Webb, R. Barraclough, and M.L. Martin Fernandez. Binding of Metastasis-associated S100A4 Protein to Non-muscle Myosin Heavy Chain in vivo Visualized by Fluorescence Resonance Energy Transfer Microscopy. European Biophysics Journal with Biophysics Letters (2005) 34 (1): 19-27.
  75. G. Wang, S. Zhang, D.G. Fernig, M.L. Martin Fernandez, P.S. Rudland, and R. Barraclough. Mutually Antagonistic Actions of S100A4 and S100A1 on Normal and Metastatic Phenotypes.  Oncogene (2005) 24 (8): 1445-1454.
  76. R.D.Perrins, M.L.Martin-Fernandez, M.J.Tobin, C.W.Wharton & R.A.Meldrum  Real-time imaging of photo-controlled DNA polymerisation. Mutation Research. Fund. & Mol Mech of Mutagenesis (2005) 577, Supp1, 573.
  77. M.L. Martin-Fernandez, S.V. Longshaw, I. Kirby, G. Santis, M.J. Tobin, D.T. Clarke, and G.R. Jones. Adenovirus Type 5 Entry and Disassembly Followed in Living Cells by FRET, Fluorescence Anisotropy, and FLIM. Biophysics Journal (2004) 87:1316-1327.
  78. Moritz W, Yoshinobu T, Finger F, F, Krause S, Martin-Fernandez M.L., Schoning MJ. High resolution LAPS using amorphous silicon as the semiconductor material. Sensor Actuat B-Chem (2004) (1-2): 436-441.
  79. G. Wang, S. Zhang, D.G. Fernig, D. Spiller, M.L. Martin Fernandez, H. Zhang, Y. Ding, Z. Rao, P.S. Rudland, and R. Barraclough. Heterodimerisation and heterodimeric interfaces of S100A1 and S100P. Biochemical Journal (2004) 382: 375-383.
  80. M.L. Martin-Fernandez, M.J. Tobin, S.V. Jones, D.T. Clarke, and G.R. Jones. Preformed Oligomeric EGF Receptors Undergo an Ectodomain Structure Change during Signalling.  Biophysics Journal (2002) 82: 2415–2427.
  81. Clarke, D.T., Seddon, E.A.; Quinn, F.M.; Clarke, J.A. Tobin, M.J., Martin-Fernandez, M.L., and Jones, G.R. 4GLS - A fourth generation light source that for the biomedical scientist is more than a laser and more than a storage ring. Biomedical Applications of Ultrafast and Free-Electron Lasers (2002). 4633: 233-242
  82. N.J. Gay, M. Symmons, M.L. Martin-Fernandez, G. Jones. Structure and Stabilisation of Self-assembling Peptide Filaments. Self-assembling Peptide Systems in Biology (2001). Kluwer Academic Publishers, pp 113-125.
  83. Martin-Fernandez, M.L., Clarke, D.T., Tobin, M.J., and Jones, G.R. Real-time studies of the interactions between epidermal growth factor and its receptor during endocytic trafficking. Cell. Mol. Biol. (2000) 46, 1103-1112.
  84. Clarke, D.T., Bowler, M., Fell, B., Flaherty, J., Grant, A., Jones, G., Martin-Fernandez, M.L., Shaw, D., Todd, B., Wallace, B. and Towns-Andrews, E.. A high aperture beamline for vacuum ultraviolet circular dichroism on the SRS. Synchrotron Radiation News (2000) 13 (2). pp. 21-27. ISSN 0894-0886. DOI: 10.1080/08940880008261066 ·
  85. Martin-Fernandez, M.L., Tobin, M.J., Clarke, D.T., Gregory, C.M., and Jones, G.R. Sub-nanosecond polarised microfluorimetry in the time domain: An instrument for studying receptor trafficking in live cells. Rev. Sci. Instrum. (1998) 69, 540-543.
  86. Tobin, M.J., Martin-Fernandez, M.L., and Jones, G.R. A facility for confocal imaging and microvolume fluorescence lifetime spectroscopy at the SRS. Synchrotron Radiation News (1998) 24-30; doi/abs/10.1080/08940889808260938. 
  87. Martin-Fernandez, M.L., Tobin, M.J., Clarke, D.T., Gregory, C.M., and Jones, G.R. A high sensitivity time-resolved microfluorimeter for real-time cell biology. Rev. Scientific Instrum. (1996) 67, 3716-3721.
  88. Martin-Fernandez, M.L., Bordas, J., Diakun, G., Harries, J., Lowy, J., Mant, G.R., Svensson, A., Towns-Andrews, E. Time-resolved X-ray-diffraction studies of myosin head movements in live frog sartorius muscle during isometric and isotonic contractions. J. Muscle Res. Cell Mot. (1994) 15, 319-348.
  89. Martin-Fernandez M.L., Diakun, G.P., Diaz F.G., Harries J.E., Lewis R.A., Lowy, Mant G., Towns-Andrews E. and Bordas, J. (1994).  X-ray-diffraction evidence for a specific actomyosin complex in live isometrically contracting frog sartorius muscle. Synchrotron. Rad. Biosciences (1994) 435-442.
  90. Martin-Fernandez M.L., Diakun, G.P., Diaz F.G., Harries J.E., Lewis R.A., Lowy, Mant G., Towns-Andrews E. and Bordas, J. X-ray-diffraction evidence for a specific actomyosin complex in live isometrically contracting frog sartorius muscle. Synchrotron. Rad. Biosciences (1994)  435-442
  91. Bordas, J., Diakun, G.P., Diaz, F.G., Harries, J.E., Lewis, R.A., Lowy, J., Mant, G.R., Martin-Fernandez, M.L., and Towns-Andrews E. Two-dimensional time-resolved X-ray-diffraction studies of live isometrically contracting frog sartorius muscle. J. Muscle Res. Cell Mot. (1993) 14, 311-324.
  92. Harries, J.E., Martin-Fernandez, M.L., Diakun, G.P., Mant, G.R., Bordas, J. A comparative-study of the supramolecular structure of frog sartorius and dorsal semitendinosus muscle. J. Structural Biology (1991) 106, 264-271.
  93. J. Bordas, G.P. Diakun, J.E. Harries, R.A. Lewis, G.R. Mant, M.L. Martin-Fernandez, E. Towns-Andrews. Two-dimensional time resolved X-ray diffraction of muscle. Advances in Biophysics (1991) Volume 27, Pages 15-33
  94. Worgan, J.S., Lewis, R., Fore, N.S., Sumner, I.L., Berry, A, Parker, B., Dannunzio, F., Martin-Fernandez, M.L., Towns-Andrews, E., Harries, J.E., Mant, G.R., Diakun, G., Bordas, J. The application of multiwire x-ray-detectors to experiments using synchrotron radiation. Nuc Ins. & Met. Phys. Res. (1990) 291, 447-454.

Conference ​papers

  1. ​Sarah R Needham, Sumanth Iyer, Benjamin Davis, Laura Zanetti Domingues, Selene K Roberts, Daniel R Rolfe, Marisa L. Martin-Fernandez.  Investigating the Structure of Epidermal Growth Factor Receptor (EGFR) Dimers and Oligomers in Cells. Biophyscal J 2021 S0006-3495(20)32965-9.pdf
  2. ​Needham, S.R, Zanetti-Domingues, L.C., Arkhipov, A., Mysore, V., Korovesis, D., Roberts, S.K., Tynan, C. J., Rolfe, D. J., Hirsch, M., Lajevardipour, A., Clayton, A.H.A., Parker, P.J., Shan, Y.E. Shaw, D. and Martin-Fernandez, M. L. (2017). Oligomerization of the Epidermal Growth Factor Receptor Organizes Kinase-Active Dimers into Competent Signaling Platforms. Biophysical J. 112. 26a-27a. 10.1016/j.bpj.2016.11.180.
  3. Webb, S.E.D., Needham, S.R., Roberts, S.K., Martin-Fernandez, M.L. Nanoscale EGFR separations in cells using fluorophore localisation confidence intervals. 9th European-Biophysical-Societies-Association Congress, Volume: 42 (2013).
  4. Tynan, C.J., Roberts, S.K., Martin-Fernandez, M.L. Human-EGFR aligned on the plasma membrane adopts key features of Drosophila-EGFR asymmetry (2011). Biochemical Society, V2, (2011).
  5. ​Askari, J. A., Tynan, C. J., Webb, S. E., Martin-Fernandez, M. L., Ballestrem, C., Humphries, M. J. (2010). Imaging integrin alpha 5 beta 1 conformation on adherent cells' International J. Exp. Path. 91 (6) ​​​

​Chapters in books

  1. ​M.L. Martin-Fernandez, David T. Clarke, Sarah R. Needham, Selene K. Roberts, Daniel J. Rolfe, Chris J. Tynan, Stephen E.D. Webb and Martyn Winn. (2011). Investigating the conformation of membrane proteins in cells via single molecule microscopy. Molecular Imaging. ISBN 979-953-307-076-5.
  2. D.T. Clarke, S.E.D. Webb, D.J. Rolfe, M.D. Winn and M.L. Martin-Fernandez. (2011). Single molecule fluorescence imaging of molecular interactions in mammalian cells In “Microscopy: Science, Technology, Applications and Education". Edt. A. Mendez-Vilas & J. Diaz.
  3. N.J. Gay, M. Symmons, M.L. Martin-Fernandez, G. Jones. Structure and Stabilisation of Self-assembling Peptide Filaments. Self-assembling Peptide Systems in Biology, Medicine and Engineering'. Kluwer Academic Publishers, pp 113-125.
  4. Nicholson, J., Martin-Fernandez, M.L., Reynolds, C., Wood, C. & Baldwin, J. (2006). Future opportunities for chromatin structure-function research on the proposed UK Fourth Generation Light Source (4GLS). In 'Chromatin Structure and Function'. Edt J. Nicholson and C. Wood: 141-152. (12 pages, 20%).
  5. ​R.D. Perrins, M.L. Martin-Fernandez, M.J. Tobin, C.W. Wharton & R.A. Meldrum (2005) Real-time imaging of photo-controlled DNA polymerisation. Mutation Research, Fundamental & Molecular Mechanisms of Mutagenesis 577, Supp1, 573. (20%)

Grant funding

2018-2023     ST/S000682/1 £535,466 - An automated macromolecular structure-based approach to personalised anti-cancer therapy​
2019-2020     BB/S019553/1 £593,518 - Bearing the context in mind: A cryo FIB-SEM based CLEM workflow to investigate relationships between molecular interactions and ultrastructure
2015-2017     STFC Innovation £248,331 - Receptor Structure for Targeted Therapeutics 
2014-2015     BB/L014327/1 £702,045 - A sharper light from gSTED microscopy 
2014-2018     MR/K015591/1 £1,700.000 - Multi-scale in vitro and in vivo imaging platform 
2009-2015     BB/G006911/1 £2,144,604 - Supra-molecular rules in signalling networks
2007-2010     SIP-STFC £300K - Lab-in-a-cell: a concept in high-throughput drug discovery 
2006-2008     BB/E000215/1 £163K - A Bayesian Algorithm for single-molecule data
2005-2008     BB/C51464X/1 £256K - Conformational dynamics in signalling EGF receptors
2003-2005     BBSRC BB/C09632 £125K – single molecule imaging of the EGFR receptor

Patents

2017       Patent Application UK 1704275.5; Cryo Super-resolution microscopy​
2016       Patent Application UK 1507202.8; US Patent App. 15/569,871 - Receptor Tyrosine Kinase Biomarkers 
2009       No. GB0707433.9; Single Molecule Fluorescence Measurement ​


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Contact: Bateman, Benji (STFC,RAL,CLF)

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